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Transcription Gene Silencing Pdf Download

 
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PostPosted: 31st October 2016, 13:09    Post subject: Transcription Gene Silencing Pdf Download Reply with quote




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Transgenes containing both endogenous and exogenous sequences, such as the nitrite reductase (Palauqui et al., 1997) and GFP (Voinnet et al., 2000) genes, can be silenced systemically through grafting. Franco-Zorrilla et al. They fall into two types of approach: protection of the plant against attack and fine-tuning of metabolic pathways. We do not capture any email address. Andrew Eamens*, Ming-Bo Wang, Neil A. Amplicons or transgene vectors were developed, consisting of a cDNA of replicating PVX RNA into which a foreign gene could be inserted. Tobacco plants inoculated with the PVX:GUS:CP amplicon accumulated virus-derived sequences at a much lower than expected level and showed no symptoms of PVX infection. Cell 75: 843854 CrossRefMedlineWeb of Science Lindbo JA, Silva-Rosales L, Proebsting WM, Dougherty WG (1993) Induction of highly specific antiviral state in transgenic plants: implications for regulation of gene expression and virus resistance.

Accepted February 26, 2008. Plant J 50: 4053 CrossRefMedline Park W, Li J, Song R, Messing J, Chen X (2002) CARPEL FACTORY, a Dicer homolog, and HEN1, a novel protein, act in microRNA metabolism in Arabidopsis thaliana. View larger version: In this window In a new window Download as PowerPoint Slide Figure 3. Although neither of these approaches have reached the commercial product stage in agronomic crops, the promise of an inexpensive and environmentally clean way of controlling plant parasitic nematodes, which are estimated to cause annual crop losses of over $125 billion worldwide (Chitwood, 2003), suggests that as long as the resistance holds true in the field, the application of such technology will only be a matter of time. Jones et al. Plant Biotechnol J 5: 802814 CrossRefMedline Napoli C, Lemieux C, Jorgensen R (1990) Introduction of a chimeric chalcone synthase gene into petunia results in reversible co-suppression of homologous genes in trans. Besides bacteria and fungi, a recent report shows that silencing of the RDR protein RDR1 in Nicotiana attenuata, either with a virus-induced silencing vector or with an IR transgene, significantly increases the susceptibility of the plant to attack by herbivorous insects such as Manduca sexta, mirids, beetles, and grasshoppers (Pandey and Baldwin, 2007).

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